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ORIGINAL ARTICLE
Year : 2010  |  Volume : 52  |  Issue : 3  |  Page : 220-228

Evidence of altered DNA integrity in the brain regions of suicidal victims of Bipolar Depression


1 Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore; Department of Applied Zoology (Presently), Mangalore University, Mangalagangothri, India
2 Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India; Department of Biochemistry and Molecular Biology (Presently), University of Texas Medical Branch, Galveston, Texas, USA
3 Malankara Orthodox Syrian Church Medical College and Hospital, Kolencherry, India
4 Institute for Scientific Research and Technology Services (INDICASAT), India
5 Institute for Scientific Research and Technology Services (INDICASAT); National Secretariat of Science, Technology and Innovation (SENACYT), City of Knowledge, Panama, India
6 Jawaharlal Nehru Technological University, Hyderabad, India
7 Department of Anatomy, JSS Medical College Hospital, M.G. Road, Mysore, India
8 Department of Psychiatry, JSS Medical College Hospital, M.G. Road, Mysore, India

Correspondence Address:
T S Sathyanarayana Rao
Department of Psychiatry, JSS Medical College Hospital, M. G. Road, Mysore, India

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Source of Support: Department of Biotechnology, India, Conflict of Interest: None


DOI: 10.4103/0019-5545.70974

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Deoxyribonucleic acid (DNA) integrity plays a significant role in cell function. There are limited studies with regard to the role of DNA damage in bipolar affective disorder (BP). In the present study, we have assessed DNA integrity, conformation, and stability in the brain region of bipolar depression (BD) patients (n=10) compared to age-matched controls (n=8). Genomic DNA was isolated from 10 postmortem BD patients' brain regions (frontal cortex, Pons, medulla, thalamus, cerebellum, hypothalamus, Parietal, temporal, occipital lobe, and hippocampus) and from the age-matched control subjects. DNA from the frontal cortex, pons, medulla, and thalamus showed significantly higher number of strand breaks in BD (P <0.01) compared to the age-matched controls. However, DNA from the hippocampus region was intact and did not show any strand breaks. The stability studies also indicated that the melting temperature and ethidium bromide binding pattern were altered in the DNA of BD patients' brain regions, except in the hippocampus. The conformation studies showed B-A or secondary B-DNA conformation (instead of the normal B-DNA) in BD patients' brain regions, with the exception of the hippocampus. The levels of redox metals such as Copper (Cu) and Iron (Fe) were significantly elevated in the brain regions of the sufferers of BD, while the Zinc (Zn) level was decreased. In the hippocampus, there was no change in the Fe or Cu levels, whereas, the Zn level was elevated. There was a clear correlation between Cu and Fe levels versus strand breaks in the brain regions of the BD. To date, as far as we are aware, this is a new comprehensive database on stability and conformations of DNA in different brain regions of patients affected with BD. The biological significance of these findings is discussed here.



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