|Year : 2013 | Volume
| Issue : 4 | Page : 371-375
Chromosome 18p11.2 harbors susceptibility marker: D18S452, for bipolar affective disorder
Mutahar Andrabi1, Arshad Hussain2, Fouzia Rashid1, Sheikh Ozair Nissar3, Idrees Ayoub Shah1, Yasir Hasan Rather2, Waseem Hassan Ahangar2, Nazir Ahmad Dar1
1 Department of Biochemistry, University of Kashmir, Jammu and Kashmir, India
2 Department of Psychiatry, Government Medical College, Srinagar, Jammu and Kashmir, India
3 Ram Manohar Lohia Hospital, New Delhi, India
Aim: The aim of our study was to investigate whether the tandem repeat polymorphism in D18S452 microsatellite marker at locus 18p11.2 is a risk factor of bipolar affective disorder (BPAD) in Kashmiri population.
Materials and Methods: The repeat polymorphism in D18S452 was evaluated by polymerase chain reaction (PCR) analysis of in 74 diagnosed BPAD patients and 74 controls subjects.
Results: Tandem repeat (300 bp*) allele frequency was found to be 1.35% in controls and 8.108% in cases. The tandem repeat (250 bp*) allele frequency was found to be in 91.89% in cases and 98.65% in controls. The 252 bp/252 bp genotype was found to be present in 89.18% of cases and 98.64% of controls, the 300 bp/300 bp genotype in 5.40% of cases and 1.35% of controls and the 252 bp/300 bp variant in 5.40% of cases and none among the controls. Although the proportion of patients homozygous for tandem repeat (300 bp/300 bp) was higher in cases than in controls, the difference was not statistically significant when 252 bp/252 bp genotype was taken as reference (odds ratio [OR]=4.4242; 95% confidence interval [CI] 0.4822-40.5924); P=0.1529). However, when the frequency of heterozygous genotype (252 bp/300 bp) was compared with 252 bp/252 bp statistical significance was observed (OR=8.0603; 95% CI 1.1112-58.4646; P=0.0383).
Conclusion: This is the first study reporting a significant association between D18S452 maker with tandem repeat polymorphism in heterozygous condition (252 bp/300 bp) and the development of BPAD in Kashmiri population.
Nazir Ahmad Dar
Department of Biochemistry, University of Kashmir, Hazratbal, Srinagar 190 006, Jammu and Kashmir
Source of Support: None, Conflict of Interest: None
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